Effects of lncRNA MIAT on cell proliferation of human non-small-cell lung carcinoma / 中国病理生理杂志

AIM:To explore the expression level of long non-coding RNA(lncRNA)myocardial infarction-as-sociated transcript(MIAT)in the tissues and cells of non-small-cell lung carcinoma(NSCLC), and to investigate the effect of MIAT on the function of NSCLC cell line.METHODS:Bioinformatic data in microarray dataset GSE19804 from Gene Expression Omnibus(GEO)were collected for analyzing the difference expression of MIAT between NSCLC tissues and normal lung tissues.Clinical and prognostic data in microarray dataset GSE 30219 from GEO were also collected for an-alyzing the correlation between the expression level of MIAT and the survival time of NSCLC patients.qPCR was applied to detect the expression of MIAT in 25 paired tumor tissues and corresponding adjacent normal tissues,normal lung epithelial HBE cell line and NSCLC A549,NCI-H266 and NCI-H1299 cell lines.The specific small interfering RNA for MIAT(si-MIAT group)or negative control sequence(si-NC group)was transfected into A549 cells,and flow cytometry,colony for-mation experiment and CCK-8 assay were employed to detect the proliferation of the cells in the 2 groups.The expression levels of cyclin D1 and cyclin-dependent kinase inhibitor 1A(CDKN1A)in the 2 groups were determined by qPCR and Western blot.RESULTS:In the GEO dataset GSE19804,the expression of MIAT in NSCLC tissues was significantly ele-vated compared with normal lung tissues(P<0.05).In the GEO dataset GSE30219,the overall survival time was signifi-cantly shorter in the patients with high expression of MIAT than the patients with low expression of MIAT(P<0.05).Fur-thermore,the levels of MIAT in both NSCLC tissues and cells were higher than those in adjacent normal tissues and normal cells(P<0.05).Compared with si-NC group,lower MIAT level,cell viability and cell colony number in si-MIAT group with statistical significance were observed(P<0.05).Meanwhile, compared with si-NC group, the expression of cyclin D1 in si-MIAT group was significantly decreased(P<0.05),and inversely,the expression of CDKN1A in si-MIAT group was significantly increased(P<0.05).CONCLUSION:There is high expression of MIAT in NSCLC tissues and NSCLC cells,and knockdown of MIAT expression inhibits NSCLC cell proliferation, which provides a potential target of targeted therapy for NSCLC.

Simultaneous determination of seven constituents in Waicha Bailing Tincture by HPLC / 中成药

AIM To eslablish an HPLC method for the simultaneous content determination of brazilin,(±) protosappanin B,hydroxysafflor yellow A,safflor yellow A,oxypeucedanin,imperatorin and isoimperatorin in Waicha Bailing Tincture (Angelicae dahuricae Radix,Sappan Lignum,Carthami Flos,etc.).METHODS The analysis of methanol extract of this drug was performed on a Agilent TC-C18 column (200 mm ×4.6 mm,5 μm),with the mobile phase comprising of methanol-0.1％ glacial acetic acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 285,403,310 nm.RESULTS Seven constituents showed good linear relationships within their own ranges (r ≥0.999 5),whose average recoveries were 96.91％-99.59％ with the RSDs of 0.81％-1.19％.CONCLUSION This stable and reliable method can be used for the quality control of Waicha Bailing Tincture.

Influences of thermal insulation at different temperatures on inflammatory response and postoperative rehabilitation in children undergoing congenital dislocation hip surgery / 中华护理杂志

Objective To observe influences of thermal insulation at different temperatures on changes of vital signs,and inflammatory factors in children with congenital dislocation of the hip joint during surgery and postoperative rehabilitation.Methods Totally 39 cases of children undergoing congenital dislocation hip surgery under general anesthesia were selected and randomly divided into 32℃,38℃ and 43℃ groups.Children patients in three groups were given forced air heating at 32℃,38℃ and 43℃,respectively.The body temperature and other vital signs of each group were recorded immediately after anesthesia induction and intubation (T0),during surgical biopsy (T1),at 1h after operation(T2),2 h after operation(T3) and at the end of operation(T4).The venous blood was taken at T0 and T4 to determine the serum TGF-β activation,TNF-α,IL-1β and IL-10.Results Compared with T0,the temperature of three groups increased at T1-4 (P＜0.05),and 38℃ group had the most reliable temperature maintenance.Compared with 32℃ and 43℃ groups,serum TNF-α and IL-1β significantly decreased and TGF-β and IL-10 significantly increased in 38℃ group at T4(P＜0.05).Children in 43℃ group developed more cases of fever compared with other two groups,and the difference was significant (P＜0.05).Conclusion Temperatures setting at 32℃,38℃ and 43℃ can guarantee stable vital signs during surgery,and address hypothermia before surgery and during anesthesia induction.Applying 38℃ gas heating can maintain normal intra-operative body temperature and effectively inhibit inflammatory response.

Effects of Flavonoids of Rhizoma Drynariae on Formation of Blood Vessels in Induced Membrane by Masquelet Technique / 广州中医药大学学报

Objective To explore the effects of flavonoids of Rhizoma Drynariae on the formation of blood vessels in the induced membrane by Masquelet technique. Methods Seventy-two SD rats were randomly divided into 4 groups,namely model group,and high-,middle-and low-dose drug groups,18 rats in each group. Rat model of critical- sized femoral defect was built,and then polymethyl methacrylate (PMMA)bone cement spacer was inserted into the bone defect to induce the formation of membrane. From the first day after surgery , the rats in high-,middle-and low-dose drug groups were given gastric gavage of 0.44,0.22,0.11 g·kg-1·d-1 of Rhizoma Drynariae flavonoids, respectively, and the rats in the model group were given the same volume of normal saline. After 6-week medication,the pathologic features of bone cement- induced membrane were observed by Haematoxilin-Eosin(HE)staining,the contents of transforming growth factor(TGF)-β1 and vascular endothelial growth factor(VEGF)proteins in the induced membrane were tested by enzyme-linked immunosorbent assay (ELISA),and the mRNA levels of TGF-β1 and VEGF in the induced membrane were determined by real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Results More blood vessels in the induced membrane of the high-dose group were found than those of the other groups under the light microscope. The protein and mRNA expression levels of TGF-β1 and VEGF in the induced membrane of the 3 drug groups were much higher than those of the model group(P < 0.05). Except for the VEGF mRNA expression level, the changes of other indexes were dose-dependent. Conclusion Flavonoids of Rhizoma Drynariae are effective on enhancing the protein and mRNA expression levels of TGF-β1 and VEGF in the induced membrane, and can accelerate the vascularization,which promotes the reconstruction of bone defect.

Influences of thermal insulation at different temperatures on inflammatory response and postoperative rehabilitation in children undergoing congenital dislocation hip surgery / 中华护理杂志

Objective To observe influences of thermal insulation at different temperatures on changes of vital signs,and inflammatory factors in children with congenital dislocation of the hip joint during surgery and postoperative rehabilitation.Methods Totally 39 cases of children undergoing congenital dislocation hip surgery under general anesthesia were selected and randomly divided into 32℃,38℃ and 43℃ groups.Children patients in three groups were given forced air heating at 32℃,38℃ and 43℃,respectively.The body temperature and other vital signs of each group were recorded immediately after anesthesia induction and intubation (T0),during surgical biopsy (T1),at 1h after operation(T2),2 h after operation(T3) and at the end of operation(T4).The venous blood was taken at T0 and T4 to determine the serum TGF-β activation,TNF-α,IL-1β and IL-10.Results Compared with T0,the temperature of three groups increased at T1-4 (P＜0.05),and 38℃ group had the most reliable temperature maintenance.Compared with 32℃ and 43℃ groups,serum TNF-α and IL-1β significantly decreased and TGF-β and IL-10 significantly increased in 38℃ group at T4(P＜0.05).Children in 43℃ group developed more cases of fever compared with other two groups,and the difference was significant (P＜0.05).Conclusion Temperatures setting at 32℃,38℃ and 43℃ can guarantee stable vital signs during surgery,and address hypothermia before surgery and during anesthesia induction.Applying 38℃ gas heating can maintain normal intra-operative body temperature and effectively inhibit inflammatory response.

Analysis of the relationship between hepatitis B virus precore and basal core promoter mutations and acute-on-chronic liver failure / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To analyze the relationship between hepatitis B virus (HBV) precore (PC) and basal core promoter (BCP) mutations and HBV-related acute-on-chronic liver failure (HB-ACLF).</p><p><b>METHODS</b>Forty-four patients with HB-ACLF and 28 patients with chronic hepatitis B (CHB; used as controls) were enrolled and venous blood samples were collected from all individuals. The PC and BCP gene fragments were amplified by nested PCR. HBV genotype and BCP/PC mutations were determined by direct sequencing and analysis by BioEdit (version 7.0.9.0). Ten of the HB-ACLF patients were selected for follow-up (range: 2-8 weeks), which included once weekly sera collection to determine the relation of mutations and treatment response. Serum levels of HBV DNA were measured by real-time PCR assay, and alanine aminotransferase, total bilirubin, creatinine and albumin were measured by standard biochemical assay and used to determine the MELD score.</p><p><b>RESULTS</b>All 44 HB-ACLF patients were infected with HBV genotype C. In the CHB group, 26 patients were infected with genotype C and two with genotype B. Single mutations (A1762T, G1764A, T1753V, G1896A, and G1899A) and combined mutations (A1762T + G1764A, G1896A + G1899A, T1753V+ A1762T + G1764A, G1896A + G1899A + A1762T + G1764A, and A1762T + G1764A + G1896A) were more frequently detected in HB-ACLF patients than in CHB patients (P less than 0.05). A significantly higher proportion of PC/BCP wild-type sequences was found in patients with CHB than in patients with HB-ACLF (17.9% vs. 2.3%; x² = 5.440, P = 0.020). The proportion of patients carrying both PC and BCP mutations was significantly higher in HB-ACLF patients than in CHB patients (79.6% vs. 39.3%; x² = 12.021, P = 0.001). The proportion of patients carrying only BCP mutation was 42.9% in the CHB group and 20.5% in the HB-ACLF group (x² = 4.157, P = 0.041). No occurrences of only PC mutation were detected in either the CHB or HB-ACLF group. The combined mutations were present in all 10 of the HB-ACLF follow-up patients. Mutations G1899A, T1753V, and A1846T were correlated with disease recovery. Significant decreases in the MELD score were accompanied by decreases in the A1846T mutation.</p><p><b>CONCLUSION</b>Significantly more HB-ACLF patients carried HBV with mutations in the PC and BCP than CHB patients. Moreover, more HB-ACLF patients carried HBV with PC + BCP combined mutations and PC mutation only. The G1899A, T1753C, and A1846T mutations were associated with HB-ACLF response to treatment and improvement in liver function.</p>

The discrepancy of HBsAg titre and HBV DNA in patients with chronic hepatitis B, HBV-related liver cirrhosis and hepatocellular carcinoma / 中华肝脏病杂志

<p><b>OBJECTIVE</b>To investigate the discrepancy of HBsAg titre and correlation of HBV DNA levels among patients with chronic hepatitis B (CHB), HBV-related liver cirrhosis (LC) and hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>HBsAg titre and HBV DNA in serum samples were measured among 47 CHB, 72 LC and 54 HCC cases using Abbott chemiluminescence and fluorescence quantitative PCR, respectively. Statistical analyses among multiple groups, between two groups and about the correlation were performed using Kruskal-Wallis test, Mann-Whitney U test and Spearman test, respectively.</p><p><b>RESULTS</b>The median HBsAg titre level in serum samples decreased from 2361.10 IU/ml in CHB cohort to 1001.64 IU/ml in LC cohort and 594.35 IU/ml in HCC cohort, suggesting a statistically significant difference (x2 = 24.394, P less than 0.05). Moreover, HBsAg titre in CHB group was significantly higher than that in LC group ( Z = -3.754, P less than 0.05). CHB patients had significantly higher HBsAg titre than HCC cases ( Z = -4.630, P less than 0.05). However, there was no statistically significant difference in HBsAg titre between LC and HCC group. Among HBeAg positive patients, HBsAg titre decreased from 3259.83 IU/ml in CHB group to 1077.30 IU/ml in LC group and 789.72 IU/ml in HCC group, indicating a significant difference (x2 = 15.643, P less than 0.01). Among HBeAg negative patients, HBsAg titre declined from 1669.00 IU/ml in CHB group to 1001.64 IU/ml in LC group and 582.05 IU/ml in HCC group, suggesting of a significant difference (x2 = 6.423, P less than 0.05). Positive correlation between HBsAg titre and HBV DNA was found in CHB ( r = 0.297, P less than 0.05), LC (r = 0.346, P less than 0.05) and HCC (r = 0.452, P less than 0.05), respectively.</p><p><b>CONCLUSION</b>HBsAg titre level in serum decreased progressively from CHB to LC and HCC group. There were positive correlations between HBsAg titre and HBV DNA level in CHB, LC and HCC.</p>

Correlation between fibrinogen polymorphisms and the type of cerebral infarction / 中华医学遗传学杂志

<p><b>OBJECTIVE</b>To study the association of beta-fibrinogen(Fg) gene -148 C/T and 448 G/A polymorphisms, plasma Fg concentration, molecular reactivity and the type of cerebral infarction.</p><p><b>METHODS</b>Gene polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism. The plasma Fg concentration and the molecular reactivity were also determined.</p><p><b>RESULTS</b>The Fg concentration in MCI patients with T -148 allele was higher than that in PCI patients and controls. The MCI patients with A448 allele had higher Fg concentration, FMPV and FMPV/Amax when compared with controls, and had higher FMPV/Amax when compared with PCI patients.</p><p><b>CONCLUSION</b>FgB beta -148 and 448 mutational genotypes have impact on Fg concentrationì and therefore increase the risk of MCI.</p>

A cross-sectional study on angiotensin-converting enzyme and angiotensin II type I receptor gene polymorphism and cerebral infarction / 中华流行病学杂志

<p><b>OBJECTIVE</b>To explore the relation of angiotensin-converting enzyme (ACE) gene polymorphism, angiotensin II type I receptor (ATIR) gene polymorphism and other factors on cerebral infarction.</p><p><b>METHODS</b>One thousand three hundred fifty-one subjects from Tangshan coalmine were enrolled with study method of cluster sampling. Face to face interviews were conducted to fill in questionnaires by trained interviewers. ACE gene, ATIR gene and inflammation factors including tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), IL-8, IL-10, C reactive protein (CRP), fibrinogen (Fg), fibrin monome polymerized velocity (FMPV), absorbance maximum (A(max)), FMPV/A(max), were measured.</p><p><b>RESULTS</b>No different prevalence rates of ACE genotype were found on cerebral infarction. The distributions of AA genotype of ATIR gene in the cerebral infarction was higher than that of the controls. The prevalence of AA genotype was higher than other groups, but the prevalence of combined genotype did not show much difference. Under the existence of factors that related to cerebral infarction, AA genotype frequencies were higher than those of non-smoking and with hypertension. IL-6, ATIR gene polymorphism, sex, FMPV/A(max) were strongly related to cerebral infarction. The level of IL-6 was higher than the normal ones.</p><p><b>CONCLUSIONS</b>The prevalence of cerebral infarction obviously increased in the hypertensive groups having AA genotype of ATIR gene. In the cerebral infarction groups, the level of IL-6 was higher than that in the normal population, indicating that these can be resulted from local inflammation and immunity reactivity. Environmental and genetic factors in the pathogenesis of cerebral infarction might have coordinating functions.</p>

Changes of pulmonary Water Content in Experimental Animals During Early phase of Hypoxia / 第三军医大学学报

This paper is to report our results of the observation on the changes of the pulmonary water content of the rats and mice after their exposure to a simulated altitude of 6000 meters above sea level for seven days.It was found that the changes of the pulmonary water content varied with the duration of exposing to the high altitude. It was lower than the control value on the first day of exposure, and then it increased approaching or even being a little higher than the control value on the second and third day. But it decreased and was below the control value again from the fourth day to the seventh day. The lung weight was increasing continuously in the same period.In addition, there were progressive increase of both the wet-lung/body and dry-lung/body indices, progressive decrease of left/right ventricles ratio, and gradual rising of hemoglobin in the animals studied.

Effect of Decreased plasma Colloid Osmotic pressure on Lung Water Content in Rats under simulated High Altitude / 第三军医大学学报

Rats were made to bleed and about 40% of the total blood volume was lost. A replacement of Ringer's solution of the volume four times the lost blood volume was given and the animals were closely monitored for 24 hours. The hemoglobin level of the animals was low throughout the course of observation. The plasma colloid osmotic pressure reached the lowest point 15 minutes after bleeding, and then gradually rising up returned to a level about 90% of the control value at the end of 24 hours. The relativity between the plasma colloid osmotic pressure and the lung water content was quite significant in those rats in a low altitude environment (P0.05).The result indicates that the increased lung water content due to decreased plasma osmotic pressure could not be made further worse by hypoxia due to high altitude. The characteristic pulmonary hemodynamic changSs caused by hypoxia might be considered as the explanation of the phenomenon.